On January 22, 1992, Hoss, Gerhard; Pappert, Gunter; Schmidt, Axel published a patent.Safety of 2-Methylisothiazol-3(2H)-one hydrochloride The title of the patent was Preservation of diagnostic test reagents and kits. And the patent contained the following:
Diagnostic test kits and the reagents therein are preserved from microbial degradation by a combination of ≥2 of 2-methyl-4-isothiazolin-3-one-HCl, 2-hydroxypyridine N-oxide, chloroacetamide, N,N-methylenebis[N-(1-hydroxymethyl)-2,5-dioxo-4-imidazolidinyl]urea (I), and 5-bromo-5-nitro-1,3-dioxane. Thus, the reagents in a kit for automated determination of albumin (succinate buffer, pH 4.2; bromcresol green; Brij 35; and preservatives 2-hydroxypyridine N-oxide and I) were incubated with a mixture of bacteria, fungi, and yeasts for 6 wk at 23°. After this time, the bacterial count was reduced by 1.6 x 104 and the count of fungi and yeasts by 8.3 x 104. The experimental process involved the reaction of 2-Methylisothiazol-3(2H)-one hydrochloride(cas: 26172-54-3).Safety of 2-Methylisothiazol-3(2H)-one hydrochloride
The Article related to preservative diagnostic reagent solution, Biochemical Methods: Reagents and other aspects.Safety of 2-Methylisothiazol-3(2H)-one hydrochloride
Referemce:
Isothiazole – Wikipedia,
Isothiazole – ScienceDirect.com