On June 20, 2017, Bao, Xingyan; Luo, Weixiao; Tan, Baiqing; Li, Zhiming published a patent.Reference of 2-Methylisothiazol-3(2H)-one hydrochloride The title of the patent was Reagent for detecting glucose by hexokinase method with high stability and low cost. And the patent contained the following:
The present invention relates to the field of detection technol. for detecting blood GLU by hexokinase method, and provides liquid dual reagent composed of reagent 1 and reagent 2. Reagent 1 mainly includes buffer solution, Mg2+, ionic equilibrium agent, preservative, NAD (oxidized coenzyme I), and protective agent, and surfactant, and reagent 2 includes buffer solution, protective agent, surfactant, preservative, hexokinase (HK), glucose-6-phosphate dehydrogenase (G6P-DH) and ATP-Na2. The invention targetedly adds various protective agents including polyethylene glycol, Tween-80 and FAD to ensure stability of enzymes in reagent, adds various surfactants in order to reduce cost, particularly usage of enzymes, and to improve emulsification to enzymes and use efficiency of enzymes, thus greatly reduces reagent cost, and is very convenient for application and promotion clin. The experimental process involved the reaction of 2-Methylisothiazol-3(2H)-one hydrochloride(cas: 26172-54-3).Reference of 2-Methylisothiazol-3(2H)-one hydrochloride
The Article related to blood glucose hexokinase dehydrogenase blood diagnostic reagent, Biochemical Methods: Reagents and other aspects.Reference of 2-Methylisothiazol-3(2H)-one hydrochloride
Referemce:
Isothiazole – Wikipedia,
Isothiazole – ScienceDirect.com