On June 22, 2018, Luo, Weixiao; Gan, Yiwu; Hu, Xiaofei; Bao, Xingyan; Liu, Jiali published a patent.Reference of 2-Methylisothiazol-3(2H)-one hydrochloride The title of the patent was Reagent and method for detecting blood potassium ion by apotryptophanase. And the patent contained the following:
The invention relates to the tech. field of blood pyruvate detection, and particularly to an optimized pyruvate detection reagent with high sensitivity. The reagent comprises reagent 1, reagent 2 and reagent 3. The reagent 1 comprises buffer (tris(hydroxymethyl)aminomethane hydrochloride), EDTA·Na2, sodium chloride, protectant, tryptophan, LDH, NADH, surfactant (lauryldimethylamine oxide), and preservative. The reagent 2 comprises buffer, EDTA·Na2, sodium chloride, protectant, apotryptophanase, and preservative. The reagent 3 comprises potassium chloride, sodium chloride, Triton-100 and preservative. By specifically selecting corresponding interference-removing components for different interfering substances, the reagent has ensured accuracy and specificity, good stability and low cost. The experimental process involved the reaction of 2-Methylisothiazol-3(2H)-one hydrochloride(cas: 26172-54-3).Reference of 2-Methylisothiazol-3(2H)-one hydrochloride
The Article related to blood potassium ion apotryptophanase spectrometry, Biochemical Methods: Spectral and Related Methods and other aspects.Reference of 2-Methylisothiazol-3(2H)-one hydrochloride
Referemce:
Isothiazole – Wikipedia,
Isothiazole – ScienceDirect.com