On March 1, 2021, Wei, Zhengxi; Fang, Yuhong; Gosztyla, Maya L.; Li, Andrew J.; Huang, Wenwei; LeClair, Christopher A.; Simeonov, Anton; Tao, Dingyin; Xia, Menghang published an article.HPLC of Formula: 26172-54-3 The title of the article was A direct peptide reactivity assay using a high-throughput mass spectrometry screening platform for detection of skin sensitizers. And the article contained the following:
Chem.-peptide conjugation is the mol. initiating event in skin sensitization. The OECD test guideline uses a high-performance liquid chromatog./UV (HPLC/UV) detection method to quantify chem.-peptide conjugation in a direct peptide reactivity assay (DPRA), which measures the depletion of two synthetic peptides containing lysine or cysteine residues. To improve assay throughput, sensitivity and accuracy, an automated 384-well plate-based RapidFire solid-phase extraction (SPE) system coupled with tandem mass spectrometry (MS/MS) DPRA was developed and validated in the presence of a newly designed internal standard Compared to the HPLC/UV-based DPRA, the automated SPE-MS/MS-based DPRA improved throughput from 16 min to 10 s per sample, and substrate peptides usage was reduced from 100 mM to 5μM. When implementing the SPE-MS/MS-based DPRA into a high-throughput platform, we found 10 compounds that depleted lysine peptide and 24 compounds that depleted cysteine peptide (including 7 unreported chems. from 55 compounds we tested) in a concentration-response manner. The adduct formation between cysteine and cinnamic aldehyde and ethylene glycol dimethacrylate were further analyzed using high-performance liquid chromatog. time-of-flight mass spectrometry (HPLC-TOF-MS) to confirm the conjugation. Overall, the automated SPE-MS/MS-based platform is an efficient, economic, and accurate way to detect skin sensitizers. The experimental process involved the reaction of 2-Methylisothiazol-3(2H)-one hydrochloride(cas: 26172-54-3).HPLC of Formula: 26172-54-3
The Article related to skin sensitizer spe msms based dpra, direct peptide reaction assay (dpra), high throughput mass spectrometry, rapidfire-ms/ms, Toxicology: Chemicals (Household, Industrial, General) and other aspects.HPLC of Formula: 26172-54-3
Referemce:
Isothiazole – Wikipedia,
Isothiazole – ScienceDirect.com