On April 12, 2007, Kyriatsoulis, Apostolos; Feldmann, Susanne; Huber, Erasmus; Kobold, Uwe; Puhlmann, Angela; Von Proff, Leopold; Waechter, Nicole published a patent.Synthetic Route of 26172-54-3 The title of the patent was Release reagent for vitamin D compounds. And the patent contained the following:
The present invention concerns a reagent composition for releasing vitamin D compounds bound to vitamin D-binding protein, a method for the detection of a 25-hydroxyvitamin D compound in which the 25-hydroxyvitamin D compound is released from vitamin D-binding protein using this reagent and the mixture obtained in this manner is analyzed, the use of the reagent to release vitamin D compounds, as well as a kit for detecting 25-hydroxyvitamin D which contains the reagent for releasing vitamin D compounds in addition to the usual immunol. reagents. 25-Hydroxyvitamin D3-3-hemisuccinate-keyhole hemocyanin conjugates were prepared and used to generate antibodies in sheep. An affinity chromatog. stationary phase was prepared to purify the antibodies. Immunoassay reagents were prepared and used to determine 25-hydroxyvitamin D in serum samples from humans of Caucasian descent and of African descent. When release reagent contained 10 % DMSO, DMF, or N-methylpyrrolidone, the values from the immunoassay correlated very well with values from LC-MS-MS and were independent of the phenotype of the vitamin D-binding protein. The experimental process involved the reaction of 2-Methylisothiazol-3(2H)-one hydrochloride(cas: 26172-54-3).Synthetic Route of 26172-54-3
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Referemce:
Isothiazole – Wikipedia,
Isothiazole – ScienceDirect.com