Computed Properties of C14H10O3. Recently I am researching about F-19 NMR; SECONDARY STRUCTURES; TAR RNA; NUCLEOTIDES; DYNAMICS; EQUILIBRIUM; OLIGORIBONUCLEOTIDES; DEPROTECTION; RELAXATION; TETRALOOP, Saw an article supported by the Austrian Science Fund FWFAustrian Science Fund (FWF) [P27947, P31691, F8011-B, P32773]; Austrian Research Promotion Agency FFG [West Austrian] [BioNMR 858017]; Wiener Wissenschafts-, Forschungs-und Technologiefonds [WWTF LS17-003]. Published in ROYAL SOC CHEMISTRY in CAMBRIDGE ,Authors: Himmelstoss, M; Erharter, K; Renard, E; Ennifar, E; Kreutz, C; Micura, R. The CAS is 93-97-0. Through research, I have a further understanding and discovery of Benzoic anhydride
New RNA modifications are needed to advance our toolbox for targeted manipulation of RNA. In particular, the development of high-performance reporter groups facilitating spectroscopic analysis of RNA structure and dynamics, and of RNA-ligand interactions has attracted considerable interest. To this end, fluorine labeling in conjunction with F-19-NMR spectroscopy has emerged as a powerful strategy. Appropriate probes for RNA previously focused on single fluorine atoms attached to the 5-position of pyrimidine nucleobases or at the ribose 2 ‘-position. To increase NMR sensitivity, trifluoromethyl labeling approaches have been developed, with the ribose 2 ‘-SCF3 modification being the most prominent one. A major drawback of the 2 ‘-SCF3 group, however, is its strong impact on RNA base pairing stability. Interestingly, RNA containing the structurally related 2 ‘-OCF3 modification has not yet been reported. Therefore, we set out to overcome the synthetic challenges toward 2 ‘-OCF3 labeled RNA and to investigate the impact of this modification. We present the syntheses of 2 ‘-OCF3 adenosine and cytidine phosphoramidites and their incorporation into oligoribonucleotides by solid-phase synthesis. Importantly, it turns out that the 2 ‘-OCF3 group has only a slight destabilizing effect when located in double helical regions which is consistent with the preferential C3 ‘-endo conformation of the 2 ‘-OCF3 ribose as reflected in the (3)J (H1 ‘-H2 ‘) coupling constants. Furthermore, we demonstrate the exceptionally high sensitivity of the new label in F-19-NMR analysis of RNA structure equilibria and of RNA-small molecule interactions. The study is complemented by a crystal structure at 0.9 angstrom resolution of a 27 nt hairpin RNA containing a single 2 ‘-OCF3 group that well integrates into the minor groove. The new label carries high potential to outcompete currently applied fluorine labels for nucleic acid NMR spectroscopy because of its significantly advanced performance.
Computed Properties of C14H10O3. Bye, fridends, I hope you can learn more about C14H10O3, If you have any questions, you can browse other blog as well. See you lster.
Reference:
Isothiazole – Wikipedia,
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