Recommanded Product: 93-02-7. Recently I am researching about GREEN FLUORESCENT PROTEIN; INTRAMOLECULAR PROTON-TRANSFER; AGGREGATION-INDUCED EMISSION; QUANTUM-CHEMICAL BENCHMARK; ELECTRONIC-PROPERTIES; PHOTOPHYSICS; DYNAMICS; STATES, Saw an article supported by the National Basic Research Program of ChinaNational Basic Research Program of China [2015CB931801]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [51473093, 21404070]. Published in AMER CHEMICAL SOC in WASHINGTON ,Authors: Deng, HP; Yu, CY; Yan, DY; Zhu, XY. The CAS is 93-02-7. Through research, I have a further understanding and discovery of 2,5-Dimethoxybenzaldehyde
The tremendous gap of fluorescence emission of synthetic green fluorescent protein (GFP) chromophore to the protein itself makes it impossible to use for applications in molecular and cellular imaging. Here, we developed an efficient methodology to enhance the photoluminescence response of synthetic GFP chromophore analogues by constructing dual-self-restricted chromophores. Single self-restricted chromophores were first generated with 2,5-dimethoxy substitution on the aromatic ring, which were further modified with phenyl or 2,5-dimethoxy phenyl to form dualself-restricted chromophores. These two chromophores showed an obvious solvatofluorochromic color palette across blue to yellow with a maximum emission Stokes shift of 95 nm and dramatically enhanced fluorescence emission in various aprotic solvents, especially in hexane, where the QY reached around 0.6. Importantly, in acetonitrile and dimethyl sulfoxide, the fluorescence QYs of both chromophores were over 0.22, which were the highest reported so far in high polar organic solvents. Meanwhile, the fluorescence lifetimes also improved obviously with the maximum of around 4.5 ns. Theoretical calculations revealed a more favorable Miilliken atomic charge translocation over the double-bond bridge and illustrated much higher energy barriers for the Z/E photoisomerization together with larger bond orders compared with the GFP core chromophore, p-HBDI. Our work significantly improved the fluorescence emission of synthetic GFP chromophore analogues in polar solvents while reserved the multicolor emitting function, providing a solid molecular motif for engineering high-performance fluorescent probes.
Recommanded Product: 93-02-7. About 2,5-Dimethoxybenzaldehyde, If you have any questions, you can contact Deng, HP; Yu, CY; Yan, DY; Zhu, XY or concate me.
Reference:
Isothiazole – Wikipedia,
,Isothiazole – ScienceDirect.com